Harper JK, Arif AM, Ford EJ, Strobel GA, Porco JA, Tomer DP, Oneill KL, Heider EM, Grant DM (2003) Pestacin: a 1,3-dihydro isobenzofuran from Pestalotiopsis microspora possessing antioxidant and antimycotic activities.
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Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Evolution 39(4):783–791įill TP, Da Silva BF, Rodrigues-Fo E (2010) Biosynthesis of phenylpropanoid amides by an endophytic Penicillium brasilianum found in root bark of Melia azedarach. Front Pharmacol 4:177įelsenstein J (1985) Confidence limits on Phylogenies: an approach using the bootstrap.
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Appl Microbiol Biotechnol 86(5):1293–1312Įkor M (2014) The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Food Technol 48(4):33ĭu H, Huang Y, Tang Y (2010) Genetic and metabolic engineering of isoflavonoid biosynthesis. J Ethnopharmacol 94(1):1–23ĭorko C (1994) Antioxidants used in foods. J Ethnopharmacol 78(2–3):165–170ĭhawan K, Dhawan S, Sharma A (2004) Passiflora: a review update. Org Biomol Chem 5(13):2010–2026ĭhawan K, Kumar S, Sharma A (2001) Anti-anxiety studies on extracts of Passiflora incarnata Linneaus. Methods Enzymol 459:49–78Ĭox RJ (2007) Polyketides, proteins and genes in fungi: programmed nano-machines begin to reveal their secrets. Crit Rev Food Sci Nutr 38(6):421–464Ĭox RJ, Simpson TJ (2009) Fungal type I polyketide synthases. Chem Nat Compd 49(3):523–525Ĭhung KT, Wong TY, Wei CI, Huang YW, Lin Y (1998) Tannins and human health: a review. Microb Ecol 53(1):12–19Ĭheng MJ, Wu MD, Chen JJ, Hsieh SY, Yuan GF, Chen IS, Chang CW (2013) Secondary metabolites from the endophytic fungus of Annulohypoxylon ilanense. Acta Amaz 46(1):69–72Ĭastillo UF, Browne L, Strobel G, Hess WM, Ezra S, Pacheco G, Ezra D (2007) Biologically active endophytic streptomycetes from Nothofagus spp and other plants in Patagonia. LWT Food Sci Technol 28(1):25–30Ĭarvalho JM, Paixão LKO, Dolabela MF, Marinho PSB, Marinho AMR (2016) Phytosterols isolated from endophytic fungus Colletotrichum gloeosporioides (Melanconiaceae). Eletron J Biotechnol 3(1):40–65īrand-Wiliams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. ISRN Pharmacol 2011:619395Īzevedo JL, Maccheroni Jr, Pereira JO, Araújo WL (2000) Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Copiadora "Luiz de Queiroz", Piracicaba.Īrora DS (2011) Chandra P (2011) Antioxidant activity of Aspergillus fumigatus. In: Guia Prático: Isolamento e caracterização de microrganismos endofíticos, 1st ed. Plant Pathol Quar 5(2):74–81Īraújo WL, Lacava PT, Marco J, Lima AOS, Sobral JK, Azevedo JL, Pizzirani-Kleiner AA (2010) Isolamento de microrganismos endofíticos. incarnata endophytes.Īamir S, Sutar S, Singh SK, Baghela A (2015) A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. The study shows a great underexplored potential for industrial application of P. The phytochemical screening showed that all analyzed fungi were able to produce a kind of secondary metabolite (phenols, flavonoids, tannins and/or saponins). Four-methoxymethylphenol (4.79%), sorbicillin (33.59%) and ergosterol (23.08%) was produced by Trichoderma longibrachiatum and isopropenyl-1,4-dimethyl-1,2,3,3a,4,5,6,7-octahydroazulene were found in two Fusarium oxysporum strains.
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The chromatographic analysis of the ethyl acetate fraction from the Aspergillus strain revealed the production of orcinol (3.19%).
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Similarly, a Chaetomium strain exhibited 93.6% and 94.7% of antioxidant activity in their ethyl acetate and butanolic fractions, respectively. dentatus (former Emericella dentata) was able to produce tannins as well its butanolic extract was very similar than the BHT (butylated hydroxytoluene) (94.3% × 94.32%) and Rutin (95.8%) reference substances in the DPPH radical scavenging. Five isolates significantly produced flavonoids and phenolic compounds in the ethyl acetate and n-Butanol extracts, also saponins and high antioxidant activity against the DPPH (2.2-diphenyl-1-picrylhydrazyl) free radical. The promising fungi were evaluated specifically on their potential to produce phenolic compounds, flavonoids and for antioxidant activity. incarnata leaves, 60 were selected to determinate presence of chemical constituents related with antioxidant activity, based on their production of soluble pigments. In this study, from 315 endophytic fungi isolated from P. Passiflora incarnata, widely employed by the pharmaceutical industry, shows therapeutic effects on anxiety, nervousness, constipation, dyspepsia and insomnia based on their antioxidant compounds. Endophytes are considered one of the most important microbial resources for obtaining biomolecules of therapeutic use.